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a300 005a rrid ab 2067772  (Bethyl)


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    Bethyl a300 005a rrid ab 2067772
    A300 005a Rrid Ab 2067772, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a300+005a/BRCA2+Antibody/pmc12934461-8-7-3
    Average 94 stars, based on 88 article reviews
    a300 005a rrid ab 2067772 - by Bioz Stars, 2026-09
    94/100 stars

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    other:

    Article Title: The NORAD lncRNA assembles a topoisomerase complex critical for genome stability.
    Article Snippet: Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used RBMX – Cell Signaling (D7C2V) #14794, Lot #1, 2, (IP (6 ug/ml), western blot (1:1000), proximity ligation assay (1:250), immunofluorescence (1:250)) RBMX – Santa Cruz Biotechnology (G17) #sc-14581, Lot #K0614, (IP (6 ug/ml) and western blot (1:1000)) ALYREF – Bethyl #A302-892A, Lot #1, (IP (6 ug/ml)) ALYREF – Santa Cruz Biotechnology (11G5) #sc-32311, Lot # J1016, (western blot (1:1000)) PUM1 – Bethyl #A302-577A, Lot #1, (IP (6 ug/ml)) V5 – Abcam (SV5-Pk1) #ab27671, Lot #GR242588-4, (IP (6ug/ml)) V5 – Abcam #ab9116, Lot #GR256657-12, (western blot (1:1000)) Flag – Cell Signaling (9A3) #8146S, Lot #3, (proximity ligation assay (1:250)) TOP1 – Bethyl #A302-589A, Lot #1, (IP (6 ug/ml), proximity ligation assay (1:250)) TOP1 – Bethyl #A302-590A, Lot #3, (western blot 1:1000) TOP1 – Santa Cruz Biotechnology (C-21) #sc-32736, Lot #K0515, (western blot (1:1000)) TOP1 – Thermo Fisher Scientific (24.5) #435900, Lot #QC215904, (proximity ligation assay (1:250)) CDC5L – Bethyl #A301-681A, Lot #1, (IP (6 ug/ml)) CDC5L – Santa Cruz Biotechnology (2136C1a) #sc-81220, Lot #C0917, (western blot (1:1000)) PRPF19 – Bethyl #A300-101A, Lot #1, (western blot (1:500)) 3 nature research | reporting sum m ary April 2018 ACTB – Abcam #ab8226 (western blot (1:1000)) BRCA1 – Bethyl #A301-378A, Lot #2, (western blot (1:1000)) BRCA2 – Bethyl #A300-005A (western blot (1:1000)) Alpha-Tubulin−FITC – Sigma Aldrich #F2168 (immunofluorescence (1:1000)) Anti-Centromere antibodies (ACA) – Antibodies Incorporated #15-234-0001 (immunofluorescence (1:200)) Normal Rabbit IgG - Cell signaling #2729, Lot #8, (IP (6 ug/ml) ) Validation All antibodies were validated by western blot.

    Virus:

    Article Title: The FANCD2-FANCI heterodimer coordinates chromatin openness and cell cycle progression throughout DNA double-strand break repair.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-FANCD2 (Rabbit) Abcam Cat# ab221932; RRID: AB_3717344 Anti-FANCI (Rabbit) Invitrogen Cat# PA5-59014; RRID: AB_2643072 Anti-MRE11 (Rabbit) Novus Biologicals Cat# NB100-142; RRID: AB_350079 Anti-Histone-H4 (Rabbit) Abcam Cat# ab7311; RRID: AB_305837 Anti-BRCA1 (Mouse) Santa Cruz Biotech Cat# sc-6954; RRID: AB_626761 Anti-BLM (Rabbit) Abcam Cat# ab2179; RRID: AB_2290411 Anti-RPA (Mouse) Millipore Sigma Cat# MABE285; RRID: AB_11213221 Anti-BRCA2 (Rabbit) Bethyl Laboratories Cat# A300-005A; RRID: AB_2067772 Anti-RAD51 (Mouse) Novus Biologicals Cat# NB100-148; RRID: AB_10002131 Anti-phospho-CHK1-ser345 (Rabbit) Cell Signal Tech Cat# 2348; RRID: AB_331212 Anti-phospho-CHK2-thr68 (Rabbit) Cell Signal Tech Cat# 2661, RRID: AB_331479 Anti-phospho-H2A.X-ser139 (Rabbit) Cell Signal Tech Cat# 9718; RRID: AB_2118009 Anti-phospho-WEE1-ser642 (Rabbit) Cell Signal Tech Cat# 4910; RRID: AB_2215870 Anti-CyclinB1 (Rabbit) Cell Signal Tech Cat# 12231; RRID: AB_2783553 Anti-Rabbit-IgG (H + L) HRP Conjugate (Goat) BioRad Cat# 170–6515; RRID: AB_11125142 Anti-53BP1 (Mouse) Millipore Sigma Cat# MAB3802; RRID: AB_11212586 Anti-phospho-ATM-ser1981 (Mouse) Cell Signal Tech Cat# 4526; RRID: AB_2062663 Anti-pH3-ser10 (Mouse) Cell Signal Tech Cat# 9706; RRID: AB_331748 Goat anti-Mouse IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM Plus 594 (Goat) Invitrogen Cat# A32742; RRID: AB_2762825 Anti-Rabbit IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM Plus 647 (Goat) Invitrogen Cat# A32733; RRID: AB_2866492 Bacterial and virus strains Escherichia coli strain DH5α – N/A Escherichia coli strain STBL3 – N/A Chemicals, peptides, and recombinant proteins Cas9-NLS (Streptococcus pyogenes) UC Berkeley QB3 MacroLab N/A (Z)-4-Hydroxytamoxifen (4-OHT) Millipore Sigma Cat# H7904 ML323 (USP1 DUB Inhibitor) SelleckChem Cat# S7529 PFM01 (MRE11 Endonuclease Domain Inhibitor) Millipore Sigma Cat# SML1735 Mirin (MRE11 Exonuclease Domain Inhibitor) Millipore Sigma Cat# M9948 NSC-105808 (DNA2 Inhibitor) National Cancer Institute (NCI) Developmental Therapeutics Program (DTP) N/A KU-55933 (ATM Kinase Inhibitor) SelleckChem Cat# S1092 M4076 (Lartesertib) (ATM Kinase Inhibitor) SelleckChem Cat# E1057 AZD1390 (ATM Kinase Inhibitor) SelleckChem Cat# S8680 AZ20 (ATR Kinase Inhibitor) SelleckChem Cat# S7050 AZD6738 (Ceralasertib) (ATR Kinase Inhibitor) SelleckChem Cat# S7693 (Continued on next page) Cell Reports 45, 116830, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mitomycin C (MMC) Millipore Sigma Cat# M5353 Thymidine Millipore Sigma Cat# T1895 5-Ethynyl-2′-deoxyuridine (EdU) Vector Laboratories Cat# CCT-1149 AZDye 488 Azide Vector Laboratories Cat# CCT-1275 4′,6-Diamidino-2-Phenylindole (DAPI) Nucleic Acid Stain ThermoFisher Cat# D1306 Propidium Iodide Millipore Sigma Cat# P4170 DMEM GlutaMAX Gibco Cat# 10569044 Fetal Bovine Serum (FBS) R&D Systems Cat# S11550 Penicillin/Streptomycin (P/S) Gibco Cat# 15140122 MEM Alpha Gibco Cat# 12571071 RPMI 1640 Gibco Cat# 72400120 Puromycin Dihydrochloride Gibco Cat# A1113803 Geneticin G418 Sulfate Gibco Cat# 10131027 Opti-MEM Reduced Serum Media Gibco Cat# 31985070 Bovine Serum Albumin Fisher Scientific Cat# BP1605-100 Pierce ECL 2 Western Blotting Substrate Thermo Scientific Cat# PI80196 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Scientific Cat# 34095 Shrimp Alkaline Phosphatase (rSAP) New England Biolabs Cat# M0371 16% Formaldehyde (w/v), Methanol-free Thermo Scientific Cat# 28908 Halt Protease Inhibitor Cocktail (100X) Fisher Scientific Cat# PI78429 Proteinase K from Tritirachium album Millipore Sigma Cat# P6556 Monarch RNase A New England Biolabs Cat# T3018L Critical commercial assays TransIT-LT1 Transfection Reagent Mirus Bio Cat# MIR2305 Lipofectamine 2000 Transfection Reagent Invitrogen Cat# 11668019 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Cat# 4568096 SE Cell Line 96-well Nucleofector Kit Lonza Cat# V4SC-1096 SE Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-1024 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2024 P2 Primary Cell 4D-Nucleofector X Kit L Lonza Cat# V4XP-2024 Dynabeads Protein A for Immunoprecipitation Invitrogen Cat# 10001D Dynabeads Protein G for Immunoprecipitation Invitrogen Cat# 10003D SsoAdvanced Universal SYBR Green Supermix Bio-Rad Cat# 1725271 HiScribe® T7 Quick High Yield RNA Synthesis Kit New England Biolabs Cat# E2050S Total RNA Purification Maxi Kit Norgen BioTek Cat# 26800 RNeasy Mini Kit Qiagen Cat# 74106 MinElute PCR Purification Kit Qiagen Cat# 28004 xGenTM ssDNA & Low-Input DNA Library Preparation Kit Integrated DNA Technologies Cat# 10009817 CellTrace Violet Cell Proliferation Kit Invitrogen Cat# C34557 Click-It EdU Alexa Fluor 488 Imaging Kit Invitrogen Cat# C10337 Click-It EdU Alexa Fluor 647 Imaging Kit Invitrogen Cat# C10340 (Continued on next page) 18 Cell Reports 45, 116830, January 27, 2026

    Recombinant:

    Article Title: The FANCD2-FANCI heterodimer coordinates chromatin openness and cell cycle progression throughout DNA double-strand break repair.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-FANCD2 (Rabbit) Abcam Cat# ab221932; RRID: AB_3717344 Anti-FANCI (Rabbit) Invitrogen Cat# PA5-59014; RRID: AB_2643072 Anti-MRE11 (Rabbit) Novus Biologicals Cat# NB100-142; RRID: AB_350079 Anti-Histone-H4 (Rabbit) Abcam Cat# ab7311; RRID: AB_305837 Anti-BRCA1 (Mouse) Santa Cruz Biotech Cat# sc-6954; RRID: AB_626761 Anti-BLM (Rabbit) Abcam Cat# ab2179; RRID: AB_2290411 Anti-RPA (Mouse) Millipore Sigma Cat# MABE285; RRID: AB_11213221 Anti-BRCA2 (Rabbit) Bethyl Laboratories Cat# A300-005A; RRID: AB_2067772 Anti-RAD51 (Mouse) Novus Biologicals Cat# NB100-148; RRID: AB_10002131 Anti-phospho-CHK1-ser345 (Rabbit) Cell Signal Tech Cat# 2348; RRID: AB_331212 Anti-phospho-CHK2-thr68 (Rabbit) Cell Signal Tech Cat# 2661, RRID: AB_331479 Anti-phospho-H2A.X-ser139 (Rabbit) Cell Signal Tech Cat# 9718; RRID: AB_2118009 Anti-phospho-WEE1-ser642 (Rabbit) Cell Signal Tech Cat# 4910; RRID: AB_2215870 Anti-CyclinB1 (Rabbit) Cell Signal Tech Cat# 12231; RRID: AB_2783553 Anti-Rabbit-IgG (H + L) HRP Conjugate (Goat) BioRad Cat# 170–6515; RRID: AB_11125142 Anti-53BP1 (Mouse) Millipore Sigma Cat# MAB3802; RRID: AB_11212586 Anti-phospho-ATM-ser1981 (Mouse) Cell Signal Tech Cat# 4526; RRID: AB_2062663 Anti-pH3-ser10 (Mouse) Cell Signal Tech Cat# 9706; RRID: AB_331748 Goat anti-Mouse IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM Plus 594 (Goat) Invitrogen Cat# A32742; RRID: AB_2762825 Anti-Rabbit IgG (H + L) Highly CrossAdsorbed Secondary Antibody, Alexa FluorTM Plus 647 (Goat) Invitrogen Cat# A32733; RRID: AB_2866492 Bacterial and virus strains Escherichia coli strain DH5α – N/A Escherichia coli strain STBL3 – N/A Chemicals, peptides, and recombinant proteins Cas9-NLS (Streptococcus pyogenes) UC Berkeley QB3 MacroLab N/A (Z)-4-Hydroxytamoxifen (4-OHT) Millipore Sigma Cat# H7904 ML323 (USP1 DUB Inhibitor) SelleckChem Cat# S7529 PFM01 (MRE11 Endonuclease Domain Inhibitor) Millipore Sigma Cat# SML1735 Mirin (MRE11 Exonuclease Domain Inhibitor) Millipore Sigma Cat# M9948 NSC-105808 (DNA2 Inhibitor) National Cancer Institute (NCI) Developmental Therapeutics Program (DTP) N/A KU-55933 (ATM Kinase Inhibitor) SelleckChem Cat# S1092 M4076 (Lartesertib) (ATM Kinase Inhibitor) SelleckChem Cat# E1057 AZD1390 (ATM Kinase Inhibitor) SelleckChem Cat# S8680 AZ20 (ATR Kinase Inhibitor) SelleckChem Cat# S7050 AZD6738 (Ceralasertib) (ATR Kinase Inhibitor) SelleckChem Cat# S7693 (Continued on next page) Cell Reports 45, 116830, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mitomycin C (MMC) Millipore Sigma Cat# M5353 Thymidine Millipore Sigma Cat# T1895 5-Ethynyl-2′-deoxyuridine (EdU) Vector Laboratories Cat# CCT-1149 AZDye 488 Azide Vector Laboratories Cat# CCT-1275 4′,6-Diamidino-2-Phenylindole (DAPI) Nucleic Acid Stain ThermoFisher Cat# D1306 Propidium Iodide Millipore Sigma Cat# P4170 DMEM GlutaMAX Gibco Cat# 10569044 Fetal Bovine Serum (FBS) R&D Systems Cat# S11550 Penicillin/Streptomycin (P/S) Gibco Cat# 15140122 MEM Alpha Gibco Cat# 12571071 RPMI 1640 Gibco Cat# 72400120 Puromycin Dihydrochloride Gibco Cat# A1113803 Geneticin G418 Sulfate Gibco Cat# 10131027 Opti-MEM Reduced Serum Media Gibco Cat# 31985070 Bovine Serum Albumin Fisher Scientific Cat# BP1605-100 Pierce ECL 2 Western Blotting Substrate Thermo Scientific Cat# PI80196 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Scientific Cat# 34095 Shrimp Alkaline Phosphatase (rSAP) New England Biolabs Cat# M0371 16% Formaldehyde (w/v), Methanol-free Thermo Scientific Cat# 28908 Halt Protease Inhibitor Cocktail (100X) Fisher Scientific Cat# PI78429 Proteinase K from Tritirachium album Millipore Sigma Cat# P6556 Monarch RNase A New England Biolabs Cat# T3018L Critical commercial assays TransIT-LT1 Transfection Reagent Mirus Bio Cat# MIR2305 Lipofectamine 2000 Transfection Reagent Invitrogen Cat# 11668019 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Cat# 4568096 SE Cell Line 96-well Nucleofector Kit Lonza Cat# V4SC-1096 SE Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-1024 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2024 P2 Primary Cell 4D-Nucleofector X Kit L Lonza Cat# V4XP-2024 Dynabeads Protein A for Immunoprecipitation Invitrogen Cat# 10001D Dynabeads Protein G for Immunoprecipitation Invitrogen Cat# 10003D SsoAdvanced Universal SYBR Green Supermix Bio-Rad Cat# 1725271 HiScribe® T7 Quick High Yield RNA Synthesis Kit New England Biolabs Cat# E2050S Total RNA Purification Maxi Kit Norgen BioTek Cat# 26800 RNeasy Mini Kit Qiagen Cat# 74106 MinElute PCR Purification Kit Qiagen Cat# 28004 xGenTM ssDNA & Low-Input DNA Library Preparation Kit Integrated DNA Technologies Cat# 10009817 CellTrace Violet Cell Proliferation Kit Invitrogen Cat# C34557 Click-It EdU Alexa Fluor 488 Imaging Kit Invitrogen Cat# C10337 Click-It EdU Alexa Fluor 647 Imaging Kit Invitrogen Cat# C10340 (Continued on next page) 18 Cell Reports 45, 116830, January 27, 2026



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    Image Search Results


    PARPi-dependent MMEJ increase depends on HR. ( A ) MMEJ quantification using the reporter and experimental timeline from Fig. and in HT1080 cells following treatment with olaparib (5 µM), DNA-PKcsi (NU7441, 1 µM), or both (combo). Values are normalized to DMSO. Immunoblot of 53BP1 and histone H3 in parental HT1080 cells and isogenic TP53BP1 -KO cells. ( C ) MMEJ quantification in HT1080 parental cells and indicated isogenic 53BP1 -KO cells. Values are normalized to wild-type DMSO. Drug used: olaparib (5 µM). ( D ) Immunoblot of BRCA2 and actin in parental HT1080 cells and two isogenic BRCA2 -KO clonal lines. ( E ) MMEJ quantification in HT1080 parental cells and BRCA2 -KO clones. Values are normalized to wild-type DMSO. Drug used: olaparib (5 µM). Statistical analyses for panels (A), (C), and (E). Data represent three independent experiments, each the average of three technical replicates. Data are mean ± SEM. Statistical test, one way ANOVA with multiple comparison correction. ns: nonsignificant, * P <.05, ** P <.01, **** P <.0001.

    Journal: Nucleic Acids Research

    Article Title: PARP1 and PARP2 are dispensable for DNA repair by microhomology-mediated end-joining at double-ended DSBs

    doi: 10.1093/nar/gkaf1437

    Figure Lengend Snippet: PARPi-dependent MMEJ increase depends on HR. ( A ) MMEJ quantification using the reporter and experimental timeline from Fig. and in HT1080 cells following treatment with olaparib (5 µM), DNA-PKcsi (NU7441, 1 µM), or both (combo). Values are normalized to DMSO. Immunoblot of 53BP1 and histone H3 in parental HT1080 cells and isogenic TP53BP1 -KO cells. ( C ) MMEJ quantification in HT1080 parental cells and indicated isogenic 53BP1 -KO cells. Values are normalized to wild-type DMSO. Drug used: olaparib (5 µM). ( D ) Immunoblot of BRCA2 and actin in parental HT1080 cells and two isogenic BRCA2 -KO clonal lines. ( E ) MMEJ quantification in HT1080 parental cells and BRCA2 -KO clones. Values are normalized to wild-type DMSO. Drug used: olaparib (5 µM). Statistical analyses for panels (A), (C), and (E). Data represent three independent experiments, each the average of three technical replicates. Data are mean ± SEM. Statistical test, one way ANOVA with multiple comparison correction. ns: nonsignificant, * P <.05, ** P <.01, **** P <.0001.

    Article Snippet: Antibodies used for immunoblotting: PARP1 (diluted 1:2000, Cell Signaling Technology #9542), PARP2 (diluted 1:2000, Active Motif #39744, Antibody has been discontinued), β-Actin (diluted 1:5000, Cell Signaling Technology #8457), WRN (diluted 1:2000, Cell Signaling Technology #4666, RRID:AB_10692114 ), EXO1 (diluted 1:1000, Cell Signaling Technology #63862), PAR (diluted 1:2000, Cell Signaling Technology #87733), 53BP1 (diluted 1:2000, Novus Biologicals #NB100-304), H3 (diluted 1:5000, Cell Signaling Technology #9715), anti-mouse IgG, HRP-linked (diluted 1:5000, Cell Signaling #7076), anti-rabbit IgG, HRP-linked (diluted 1:5000, Cell Signaling #7074), BRCA2 (diluted 1:1000, Bethyl #A303-434A, RRID:AB_10952240 ).

    Techniques: Western Blot, Clone Assay, Comparison